CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞

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產(chǎn)品名稱: CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞
產(chǎn)品型號: FDC-P1
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔: 無相關(guān)文檔

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CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！

CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞的詳細(xì)介紹

CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞

ATCC^® Number:

CRL-12103?

Price:

$355.00

Designations:	FDC-P1
Depositors:	University of Virginia Patent Foundation
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Mus musculus (mouse)
Morphology:	lymphoblast
Source:	Organ: bone marrow Strain: DBA/2 Disease: normal
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:	transfection host (Nucleofection technology from Lonza)
Antigen Expression:	Thy-1.2 (24%)
Cytogenetic Analysis:	diploid
Comments:	The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68). They are dependent upton WEHI-3 conditioned medium (WEHI-3CM) for continued growth.
Propagation:	ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040). Temperature: 37.0°C
Subculturing:	Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature

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CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞

CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！

CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！