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Designations:

BC-1

Depositors:

E Cesarman

Biosafety Level:

2 [CELLS CONTAIN HERPESVIRUS ]

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

suspension

Organism:

Homo sapiens (human)

Morphology:

lymphoblast

Source:

Disease: lymphoma
Cell Type: B lymphocyte;

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:

transfection host (Roche FuGENE® Transfection Reagents)

Antigen Expression:

CD30 +; CD45 +; EMA + (epithelial membrane antigen)

DNA Profile (STR):

Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8
D16S539: 12,13
D5S818: 11,12
D7S820: 11
THO1: 9.3
TPOX: 8,9
vWA: 16,20

Age:

46 years

Gender:

male

Comments:

BC-1 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from an acquired immunodeficiency syndrome (AIDS) related body cavity based lymphoma presenting as a primary lymphomatous effusion in the peritoneal cavity.
The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).
KSHV is a recently identified and largely uncharacterized virus.
The BC-1 cell line allows in vitro culture of KSHV viral genomes.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests.

Propagation:

ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing:

Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.

Preservation:

Culture medium, 95%; DMSO, 5%

Doubling Time:

48 to 72 hrs

References:

22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
23358: Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109
23395: Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575
23493: Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311

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CRL-2230 BC-1 人lymphoma B **細胞

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