immunoglobulin; monoclonal antibody; against human T cell (human T lymphocyte) and human E rosette positive thymocytes (human thymic lymphocyte); against CD2
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Comments:
Mice were immunized with human T cell leukemia cells (acute lymphoblastic leukemia).
Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:
3507: Kung PC, Goldstein G. Monoclonal antibody to a human T cell antigen and methods of preparing same. US Patent 4,364,937 dated Dec 27 1982